FFAR1 antibody

Catalog No. ABIN290047

Product Details anti-FFAR1 Antibody

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FFAR1 antibody is un-conjugated

Application: Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purification: The splenic lymphocytes from GANP mouse, immunized with the first extracellulardomain peptides of mouse GPR40 conjugated with KLH, were fused to myeloma P3U1cells. The screening of the hybridoma cells was performed on ELISA. The cell line with positive reaction was grown on non-serum medium, from which the antibody waspurified by Protein G affinity chromatography.

Immunogen: Partial peptide of Mouse GPR40

Clone: G16

Isotype: IgG2a kappa

Application Details

Application Notes: Working dilution For Immunocytochemistry 1.0 ug/ml. For Fluorescence activated cell sorting 0.5 ug/ml

Restrictions: For Research Use only

Handling

Concentration: 0.25 mg/ml

Buffer: PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)

Preservative: ProClin

Storage: -20 °C

Target Details for FFAR1

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Alternative Name: GPR40 (FFAR1 Products)

Synonyms: FFAR1 antibody, GPR40 antibody, FFA1R antibody, GPCR40 antibody, Gpr40 antibody, free fatty acid receptor 1 antibody, FFAR1 antibody, Ffar1 antibody

Background: G protein coupled receptors (GPCRs) are a protein family of transmembrane receptors, and involved in a widevariety of physiological processes. GPR40 is also a member of GPCRs and functions as a receptor for long-chainfree fatty acids (FFA), which is expressed in beta cells in the islets of pancreas and is involved in the regulationof glucose and lipid metabolisms. Long chain FFAs amplify glucose-stimulated insulin secretion from pancreaticbeta cells by activating GPR40.GPR40 knockout mice fed a high-fat diet are protected against obesity-induced hyperinsulinemia and hepaticsteatosis. Transgenic mice overexpressed GPR40 in pancreatic beta cells had impaired beta cell function anddeveloped diabetes.GPR40 is an important mediator of both the acute, stimulatory effects and chronic,deleterious effects of FFAs on beta cells.Moreover, GPR40 may be implicated in the control of breast cancer cell growth by fatty acids and provide a linkbetween fat and cancer.This antibody is specific to GPR40 and will be useful to research for lipid metabolism, obesity and diabetes.

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Carbohydrate Homeostasis