TNFRSF10B antibody (AA 388-407)

Catalog No. ABIN290834

The Rabbit Polyclonal anti-TNFRSF10B antibody is suitable to detect TNFRSF10B in samples from Human. It has been validated for WB, FACS, IHC, ICC and IHC (p).

Quick Overview for TNFRSF10B antibody (AA 388-407) (ABIN290834)

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF10B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TNFRSF10B Antibody

Binding Specificity: AA 388-407

Specificity: A peptide corresponding to amino acids 388 to 407 (isoform 2) of human DR5 precursor. The same sequence is found in isoform 1 at amino acids 417-436.

Purification: Protein G purified

Immunogen: A peptide corresponding to amino acids 388 to 407 (isoform 2) of human DR5 precursor (1,2). The same sequence is found in isoform 1 at amino acids 417-436.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: Flo, ICC, IHC, IHC-P (10 μg/mL), WB (1:500 - 1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for TNFRSF10B

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Alternative Name: TNFRSF10B / Killer / DR5

Background: Name/Gene ID: TNFRSF10B
Family: TNF Receptor

Synonyms: TNFRSF10B, Cytotoxic TRAIL receptor-2, CD262 antigen, DR5, Death receptor 5, KILLER, TRAIL-R2, TRICK2B, TRAILR2, TRICK2A, TRICKB, TRICK2, CD262, Fas-like protein, TRAIL receptor 2, ZTNFR9

Gene ID: 8795

UniProt: O14763

Pathways: p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity