Collagen Type I antibody (Biotin)

Catalog No. ABIN2619406

The Biotin-conjugated Rabbit Polyclonal anti-Collagen Type I antibody (ABIN2619406) specifically detects Collagen Type I in WB, IHC, ELISA, IHC (p) and IP. The antibody is reactive with Human, Mouse, Rat and Cow samples.

Quick Overview for Collagen Type I antibody (Biotin) (ABIN2619406)

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Product Details anti-Collagen Type I Antibody (Biotin)

Specificity: Collagen Type I from adult human knee cartilage and bovine nasal cartilage.

Purification: Affinity purified

Immunogen:

Type of Immunogen: Native protein

Application Details

Application Notes: Approved: ELISA (1:3000 - 1:6000), IHC, IHC-P (10 μg/mL), IP, WB (1:3000 - 1:6000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.1 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA,  pH 8.0, 10 mg/mL BSA, 0.01 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen I