NEDD8 antibody

Catalog No. ABIN299488

This Rabbit Polyclonal antibody specifically detects NEDD8 in WB and ELISA. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for NEDD8 antibody (ABIN299488)

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEDD8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NEDD8 Antibody

Specificity: Anti-Rabbit Serum

Purification: Ion exchange chromatography

Immunogen: Full-length recombinant yeast Rub1 protein.

Type of Immunogen: Recombinant protein

Application Details

Application Notes: Approved: ELISA (1:1000 - 1:5000), WB (1:5000)

Usage: This purified polyclonal antibody reacts with yeast Rub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. A 6 kD band corresponding to yeast Rub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.

Comment:

Target Species of Antibody: Yeast

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze/thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

Target Details for NEDD8

Target: NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))

Alternative Name: Rub1

Pathways: Ubiquitin Proteasome Pathway