GTPase NRas (NRAS) antibody

Antigen: GTPase NRas (NRAS)

Synonyms: ALPS4, N-ras, NRAS1, AV095280, fj99a10, zras-b1, wu:fj99a10, zgc:100761, NRAS, N-RAS, MGC148418, DKFZp469K0915

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN302138

Additional Information

Alternative name: NRAS

Gene ID: 4893, 18176 (mouse), 24605 (rat)

Immunogen: Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1

Format: Affinity-purified

Description: All names and symbols: NRAS , neuroblastoma RAS viral (v-ras) oncogene homolog , N-ras , NRAS1, N-ras protein part 4 , v-ras neuroblastoma RAS viral oncogene homolog. Official Symbol: NRAS. RefSeq number(s): NP_002515.1.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 21kDa band observed in Human lysates of cell line A431 (calculated MW of 21.2kDa according to NP_002515.1). In transfected HEK293 transiently expressing NRAS bands of approx. 21kDa and 25kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03µg/ml. Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration, 2-3µg/ml. Full Caption WB pictures: A) HEK293 overexpressing NRAS (RC202681) and probed with EB08365 (mock transfection in first lane), tested by Origene. B) see Application Notes.B) EB08365 (0.01µg/ml) staining of A431 lysate (35µg protein in RIPA buffer).Primary incubation was 1 hour.Detected by chemiluminescence.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer

Restrictions: For Research Use only

Images

A) HEK293 overexpressing NRAS (RC202681) and probed with EB08365 (mock transfection in first lane), tested by Origene. B) see Application Notes.

EB08365 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Thomas, Edmiston, Alexander et al.: "Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 16, Issue 5, pp. 991-7, 2007 (PubMed).