MDM2 antibody

Catalog No. ABIN302837

The Rabbit Polyclonal anti-MDM2 antibody is suitable to detect MDM2 in samples from Human. It has been validated for WB, IF and IHC (p).

Quick Overview for MDM2 antibody (ABIN302837)

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MDM2 Antibody

Specificity: This antibody reacts to a synthetic peptide (gavecfeeedsnsresls) corresponding to amino acids 122 to 139 of MTBP, which differ from the mouse sequence by three amino acids.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide

Application Details

Application Notes: Western Blot: 0,5 - 1 μg/mL. Immunohistochemistry: 10 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Alternative Name: MDM2

Background: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, and MTBP, were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53. MTBP is expressed in a variety of normal tissues.Synonyms: Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2

Gene ID: 4193

NCBI Accession: NP_002383

UniProt: Q00987

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy, Ubiquitin Proteasome Pathway