GJB1 antibody (Middle Region)
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- Target See all GJB1 Antibodies
- GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))
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Binding Specificity
- AA 215-231, Middle Region
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Reactivity
- Human, Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GJB1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Gap junction beta-1 protein(GJB1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Sequence
- RACARRAQRR SNPPSRK
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Gap junction beta-1 protein(GJB1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: gap junction protein, beta 1, 32 kDa
Protein Name: Gap junction beta-1 protein - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence in the middle region of human Connexin 32/GJB1(215-231aa RACARRAQRRSNPPSRK), identical to the related mouse and rat sequences.
- Isotype
- IgG
- Top Product
- Discover our top product GJB1 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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- Target
- GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))
- Alternative Name
- GJB1 (GJB1 Products)
- Synonyms
- GJIC antibody, cmtx antibody, cmtx1 antibody, connexin-30 antibody, connexin32 antibody, cx30 antibody, cx32 antibody, gja1 antibody, CX32 antibody, GJB1 antibody, AI118175 antibody, Cnx32 antibody, Cx32 antibody, Gjb-1 antibody, CMTX antibody, CMTX1 antibody, gjb1-a antibody, gap junction protein beta 1 antibody, connexin 32 antibody, probable serine/threonine-protein kinase CST antibody, gap junction protein, beta 1 antibody, gap junction protein beta 1 L homeolog antibody, gjb1 antibody, CX32 antibody, LOC9303136 antibody, GJB1 antibody, Gjb1 antibody, gjb1.L antibody
- Background
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Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells(Bergoffen et al., 1993). For a general discussion of connexin proteins, also known as Gap junction beta-1 protein. Gap junction beta-1 protein is a protein that in humans is encoded by the GJB1 gene. In melanocytic cells GJB1 gene expression may be regulated by MITF.
Synonyms: Charcot Marie Tooth neuropathy X linked antibody|CMTX 1 antibody|CMTX antibody|CMTX1 antibody|Connexin-32 antibody|Connexin32 antibody|CX 32 antibody|CX32 antibody|CXB1_HUMAN antibody|GAP junction 28 kDa liver protein antibody|Gap junction beta 1 protein antibody|Gap junction beta-1 protein antibody|Gap junction protein beta 1 32kD antibody|Gap junction protein beta 1 antibody|GJB 1 antibody|GJB1 antibody - UniProt
- P08034
- Pathways
- Cell-Cell Junction Organization
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