His Tag antibody (FITC)

Details for Product No. ABIN304882, Supplier: Log in to see
Antigen
Reactivity
Tag
612
26
15
3
3
Host
Mouse
410
185
27
23
4
4
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
58
45
35
14
13
11
10
10
9
9
9
9
8
8
8
7
7
7
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
550
389
164
109
78
77
74
26
13
11
9
7
6
6
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
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Supplier Product No.
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Immunogen PAX6 transcription factor linked to histidine tag.

Type of Immunogen: Fusion protein
Sequence HHHHHH
Clone AD1-1-10
Isotype IgG1
Specificity Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian , , and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
Purification Protein G purified
Target Type Tag
Research Area Tags/Labels
Application Notes Approved: Flo (1:10)

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, 0.09 % sodium azide, 1 % BSA.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Product is photosensitive and should be protected from light. Avoid freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C
Avoid freeze-thaw cycles. Protect from light.
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