LEF1 antibody

Catalog No. ABIN306680

Product Details anti-LEF1 Antibody

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LEF1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: REMB6

Isotype: IgG1

Application Details

Application Notes: Antibody can be used for Western blotting (1-5 ug/ml) and immunofluorescence on paraformaldehyde-fixed cells (1:1000 or 1 ug/ml). Positive Control: Jurkat cell lysate.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/ml

Storage: -20 °C

Target Details for LEF1

Target: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Alternative Name: LEF/TCF/HMG (LEF1 Products)

Synonyms: LEF1 antibody, 3000002B05 antibody, AI451430 antibody, Lef-1 antibody, LEF-1 antibody, TCF10 antibody, TCF1ALPHA antibody, TCF7L3 antibody, lymphoid enhancer binding factor 1 antibody, lymphoid enhancer-binding factor 1 antibody, lymphoid enhancer factor XLEF-1B antibody, LEF1 antibody, lef1 antibody, Lef1 antibody

Background: The immunogen for the anti-LEF-1 antibody was a full length protein. Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDSPAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms. Clone REMB6 recognizes the High Mobility Group DNA domain of LEF-1Synonyms: TCF domain binding protein, mouse anti human T Cell Factor domain binding protein (includes lef-1).

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding