Caspase 8 antibody

Catalog No. ABIN306795

The Mouse Monoclonal anti-Caspase 8 antibody (Clone FLICE 1 1-37) (ABIN306795) specifically detects Caspase 8 in WB. The antibody is reactive with Human samples.

Quick Overview for Caspase 8 antibody (ABIN306795)

Target: Caspase 8 (CASP8)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: FLICE 1 1-37

Product Details anti-Caspase 8 Antibody

Purpose: Mouse anti Human Caspase-8

Cross-Reactivity: Human

Characteristics: Caspases are key effectors of programmed cell death. They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases. Caspase-8 is a key enzyme in the apoptosis pathway. Caspase-8 contains two N-terminal region death effector domains which are removed to activate the enzyme along with cleavage into the two subunits. These subunits then for the active protease which is capable of cleaving caspase-3, -6 and -7. These caspases are then capable of cleaving other cellular substrates such as PARP and DFF, which in turn induce apoptosis.

Purification: Protein A/G Chromatography

Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-8 protein and mouse myeloma cells.

Isotype: IgG

Application Details

Application Notes: Detects proenzyme form of human Caspase-8 by Western blot analysis as well as one of the cleavage products. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase-8

Background: Cysteine-requiring Aspartate Protease-8, Apoptotic protease Mch-5, ICE-like apoptotic protease 5, FADD-like ICE

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades