MUC1 antibody
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- Target See all MUC1 Antibodies
- MUC1 (Mucin 1 (MUC1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MUC1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human
- Characteristics
- Episialin,Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in >90 % breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed, is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400 kDa) and a smaller subunit (14-28 kDa) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1. Muc1 can act as a ligand for ICAM-1 on HUVEC cells, it can bind -catenin, GSK3 and it associates with Grb2-SOS upon phosphorylation.
- Purification
- Protein A/G Chromatography
- Immunogen
- Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a Muc1 60mer tandem repeat NH2-(VTSAPDTRPAPGSTAPPAHG)3-COOH and mouse myeloma SP2 cells.
- Clone
- VU-4H5
- Isotype
- IgG1
- Top Product
- Discover our top product MUC1 Primary Antibody
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- Application Notes
- Antibody can be used for immunohistochemistry on frozen and paraffin embedded tissues, ELISA and Western blotting. Optimal concentration should be evaluated by serial dilutions. Antibody can only detect Muc1 if the threonine residue of the PDTRPAP of the tandem repeat region is non-glycosylated4.
- Restrictions
- For Research Use only
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- Buffer
- Provided as solution in phosphate buffered saline with 0.08 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- Target
- MUC1 (Mucin 1 (MUC1))
- Alternative Name
- Muc1 (MUC1 Products)
- Synonyms
- CA 15-3 antibody, CD227 antibody, EMA antibody, H23AG antibody, KL-6 antibody, MAM6 antibody, MCKD1 antibody, MUC-1 antibody, MUC-1/SEC antibody, MUC-1/X antibody, MUC1/ZD antibody, PEM antibody, PEMT antibody, PUM antibody, Muc-1 antibody, mucin 1, cell surface associated antibody, mucin 1, transmembrane antibody, MUC1 antibody, Muc1 antibody
- UniProt
- P15941
- Pathways
- Negative Regulation of intrinsic apoptotic Signaling
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