Kelch-Like ECH-Associated Protein 1 (KEAP1) (C-Term) antibody

Details for Product No. ABIN309756
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Antigen
Synonyms KEAP1, INrf2, klhl19, KLHL19, INRF2, mKIAA0132, Inrf2, fb36f11, keap1, wu:fb36f11
Epitope
C-Term
(22), (8), (6), (4), (4), (2), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Pig (Porcine), Zebrafish (Danio rerio), Xenopus laevis, Human
(68), (32), (27), (15), (15), (14), (1), (1)
Host
Rabbit
(50), (14), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(59), (31), (25), (17), (11), (10), (9), (5), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN309756
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Immunogen Synthetic peptide directed towards the C terminal of human KEAP1
Sequence TWTFVAPMKH RRSALGITVH QGRIYVLGGY DGHTFLDSVE CYDPDTDTWS
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Rabbit : 90 %, Zebrafish : 90 %, African clawed frog : 80 %
Characteristics This is a rabbit polyclonal antibody against KEAP1. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name KEAP1
Background KEAP1 contains KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Molecular Weight 70 kDa
Gene ID 9817
NCBI Accession NM_203500, NP_987096
UniProt Q14145
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 624 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Kelch-Like ECH-Associated Protein 1 (KEAP1) (C-Term) antibody WB Suggested Anti-KEAP1 Antibody Titration: 1.0ug/ml
Positive Control: HepG2 cell lysate
anti-Kelch-Like ECH-Associated Protein 1 (KEAP1) (C-Term) antibody (2) anti-Kelch-Like ECH-Associated Protein 1 (KEAP1) (C-Term) antibody (Image 2)
anti-Kelch-Like ECH-Associated Protein 1 (KEAP1) (C-Term) antibody (3) Host: Rabbit
Target Name: KEAP1
Sample Tissue: HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1.25 µg/mL Peptide Concentration: 1.0 µg/mL Lysate Quantity: 241 µg/laneGel Concentration: 12 %KEAP1 is supported by BioGPS gene expression data to be expressed in HepG2
anti-Kelch-Like ECH-Associated Protein 1 (KEAP1) (C-Term) antibody (4) Human kidney
Background publications Padmanabhan, Tong, Ohta et al.: "Structural basis for defects of Keap1 activity provoked by its point mutations in lung cancer." in: Molecular cell, Vol. 21, Issue 5, pp. 689-700, 2006 (PubMed).

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