This antibody detects Apoptosis Signal-regulating Kinase 1 (ASK1) phosphorylated at serine83. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis.
Purification
Purified
Immunogen
KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
MAP3K5
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
1E3
unconjugated
Application Notes
ELISA: 1: 5,000 - 1: 10,000. Western Blot: 1: 1,000, detects a band of approximately 155 kDa in SW1353 cell lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
ASK1 is a serine/threonine kinase also known as MAP kinase kinase kinase (MAPKKK), and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5