NF-kB p65 antibody (Ser536)
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- Target See all NF-kB p65 (NFkBP65) Antibodies
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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Binding Specificity
- Ser536
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NF-kB p65 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody detects the p65 (RelA) subunit of nuclear factor kappa-B (NFkappaB) when phosphorylated at serine 536.
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Purified
- Immunogen
- Synthetic peptide corresponding to residues surrounding serine 536 of Human NFkB p65 protein.
- Isotype
- IgG
- Top Product
- Discover our top product NFkBP65 Primary Antibody
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- Application Notes
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Western Blot: 1: 100 - 1: 1000, detects a band of approximately 65 kDa in the appropriate celllysate. Immunohistochemistry on paraffin sections: 1: 50 - 1: 250. ELISA: 1: 500 - 1: 3000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- Alternative Name
- RELA / NF-kB p65 (NFkBP65 Products)
- Synonyms
- C-Rel antibody, NFKB3 antibody, p65 antibody, NFkB antibody, c-rel antibody, zgc:100833 antibody, Xrel2 antibody, Xrel3 antibody, rel antibody, rel-A antibody, rel2 antibody, rel3 antibody, v-rel antibody, xrel antibody, REL proto-oncogene, NF-kB subunit antibody, RELA proto-oncogene, NF-kB subunit antibody, v-rel reticuloendotheliosis viral oncogene homolog A (avian) antibody, v-rel avian reticuloendotheliosis viral oncogene homolog antibody, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog antibody, REL antibody, RELA antibody, Rela antibody, Rel antibody, rel antibody, rel.L antibody
- Background
- NFkappaB is a heterodimeric DNA binding protein which was originally identified consisting of p65 and p50 (NF?B1) subunits. Research has led to the discovery of other subunits, including p52 (NFkappaB2), cRel, and RelB. NFkappaB is ubiquitously expressed, and functions as a second messenger which upon activation leads to the transcription of a number of genes. NFkappaB is activated by a number of agents, including pro-inflammatory cytokines and bacterial lipopolysaccharide, and is essential for the maturation of T cells and regulation of their survival and activation. Phosphorylation of serine 536 contributes to NFkappaB transactivation and increases p65 transcription activity.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65
- Gene ID
- 5970
- NCBI Accession
- NP_001138610
- UniProt
- Q04206
- Pathways
- NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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