V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody

Details for Product No. ABIN319271
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Antigen
Synonyms
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AK ... show more
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AKT, PKB/Akt, PKB/dAKT, RAC, RacPK, akt, akt1, dAKT, dAKT/dPKB, dAKT1, dAkt, dAkt/PKB, dAkt1, dPKB, dakt, dakt1, l(3)04226, l(3)89Bq, p-Akt, pAkt, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, Rac, PKBalpha, CWS6, PKB-ALPHA, PRKBA, RAC-ALPHA show less
Epitope
pThr450
(133), (87), (64), (60), (57), (47), (36), (33), (29), (19), (18), (17), (17), (17), (16), (14), (10), (10), (9), (8), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(936), (552), (522), (164), (153), (115), (94), (24), (18), (4), (2), (1), (1), (1), (1)
Host
Rabbit
(799), (144), (10), (5), (3), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(34), (28), (27), (22), (14), (13), (13), (13), (13), (13), (13), (13), (13), (13), (7), (7), (7), (7), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(719), (431), (297), (158), (149), (132), (119), (65), (57), (41), (11), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN319271
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AKT1 around the phosphorylation site of threonine 450 (T-I-TP-P-P).
Specificity AKT1 (phospho-Thr450) Antibody detects endogenous levels of AKT1 only when phosphorylated at Threonine 450.
Purification Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name AKT1 / PKB
Background Alternate names: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Application Notes Immunohistochemistry (1/50-1/100). Immunofluorescence (1/100-1/200). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20°C.
Expiry Date 12 months
Supplier Images
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody (2) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody (Image 2)
Background publications Levin: "It's prime time for reverse transcriptase." in: Cell, Vol. 88, Issue 1, pp. 5-8, 1997 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Gesbert, Sellers, Signoretti et al.: "BCR/ABL regulates expression of the cyclin-dependent kinase inhibitor p27Kip1 through the phosphatidylinositol 3-Kinase/AKT pathway." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39223-30, 2001 (PubMed).

Salvioli, Bonafè, Capri et al.: "Mitochondria, aging and longevity--a new perspective." in: FEBS letters, Vol. 492, Issue 1-2, pp. 9-13, 2001 (PubMed).

Sarbassov, Guertin, Ali et al.: "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." in: Science (New York, N.Y.), Vol. 307, Issue 5712, pp. 1098-101, 2005 (PubMed).

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