This antibody recognises EMR2. Clone 2A1 specifically recognises the stalk region of EMR2.
Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA. Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
EMR2
Reactivity: Human
WB, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow Cytometry (Neat): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
EMR2 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family of proteins, which is closely related to CD97. EMR2, also known as CD312, is predominantly expressed on myeloid dendritic cells, monocytes and tissue macrophages. Various isoforms of EMR2 have been documented. The ligand for the largest isoform of EMR2 has recently been identified as chrondroitin sulphate, which binds to the fourth EGF-like module of EMR2.Synonyms: EGF-like module EMR2, EGF-like module-containing mucin-like hormone receptor-like 2