ITCH antibody (Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse)) (Internal Region)

Details for Product anti-ITCH Antibody No. ABIN334426, Supplier: Log in to see
Antigen
  • AIF4
  • AIP4
  • NAPP1
  • dJ468O1.1
  • 6720481N21Rik
  • 8030492O04Rik
  • A130065M08
  • C230047C07Rik
  • itchy E3 ubiquitin protein ligase
  • itchy, E3 ubiquitin protein ligase
  • ITCH
  • Itch
Alternatives
anti-Human ITCH antibody for ELISA
Epitope
Internal Region
19
11
9
9
8
5
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
62
41
11
4
4
4
3
2
1
1
1
1
1
1
Host
Goat
51
15
11
Clonality
Polyclonal
Conjugate
This ITCH antibody is un-conjugated
5
4
4
3
3
3
Method Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
53
48
10
8
6
3
3
2
1
1
Options
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Immunogen Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
Blocking Peptide Blocking peptide for this product available: ABIN368990
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ITCH / AIF4 (ITCH Antibody Abstract)
Background Synonyms: ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse), AIP4, NAPP1, dJ468O1.1, NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiquitin protein ligase ITCH
Gene ID 83737, 16396, 311567
NCBI Accession NP_113671
Pathways Activation of Innate immune Response, CXCR4-mediated Signaling Events
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 100 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7 kDa according to NP_113671.3). Recommended concentration: 0.03-0.1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-ITCH antibody (Itchy E3 Ubiquitin Protein Ligase Homolog (Mouse)) (Internal Region) (ABIN334426) ABIN334426 (0.03µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein ...
Background publications Di Marcotullio, Ferretti, Greco, De Smaele, Po, Sico, Alimandi, Giannini, Maroder, Screpanti, Gulino: "Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination." in: Nature cell biology, Vol. 8, Issue 12, pp. 1415-23, 2006 (PubMed).