Rabbit anti-Mouse IgG1 Antibody (FITC)
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- Target See all IgG1 products
- IgG1
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- FITC
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Application
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
- Specificity
- Recognizes mouse IgG1 heavy chain. Cross-reactivity against other mouse or human heavy or light chain isotypes is < 1 % . Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgG and mouse Serum.
- Purification
- Immunoaffinity purified
- Immunogen
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Mouse IgG1 gamma 1 chain.
Type of Immunogen: Purified protein - Isotype
- IgG
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- Application Notes
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Approved: ELISA, Flo (1:2000 - 1:10000), IF (1:500 - 1:2500), WB
Usage: Suitable for use in ELISA, Immunomicroscopy, Flow Cytometry, FACS analysis and fluorescent Western Blot. Also suitable for multiplex analysis. Immunomicroscopy: 1:500-1:2500. Flow Cytometry: 1:2000-1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Mouse
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- 1 mL sterile ddH20
- Concentration
- Lot specific
- Buffer
- Lyophilized powder from PBS, pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.15M sodium chloride, 0.01 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing. - Storage
- -20 °C
- Storage Comment
- Store lyophilized powder -20°C. Reconstitute with sterile ddH20, aliquot and store at -20°C.
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- Target
- IgG1
- Abstract
- IgG1 Products
- Target Type
- Antibody
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