V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody

Details for Product No. ABIN349614
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AK ... show more
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AKT, PKB/Akt, PKB/dAKT, RAC, RacPK, akt, akt1, dAKT, dAKT/dPKB, dAKT1, dAkt, dAkt/PKB, dAkt1, dPKB, dakt, dakt1, l(3)04226, l(3)89Bq, p-Akt, pAkt, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, Rac, PKBalpha, CWS6, PKB-ALPHA, PRKBA, RAC-ALPHA show less
Epitope
pSer473
(135), (90), (65), (61), (60), (49), (37), (33), (29), (20), (19), (17), (17), (17), (16), (14), (10), (10), (9), (8), (7), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(958), (558), (526), (165), (155), (117), (94), (24), (18), (4), (3), (1), (1), (1), (1)
Host
Mouse
(823), (143), (11), (5), (3), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(34), (28), (27), (22), (14), (13), (13), (13), (13), (13), (13), (13), (13), (13), (7), (7), (7), (7), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
(740), (434), (311), (162), (142), (140), (117), (70), (67), (41), (11), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 100 μg
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN349614
361.90 $
Plus shipping costs $45.00 and $26.40 dry ice

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Clone 17F6-B11
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Monkey
Characteristics Concentration Definition: by UV absorbance at 280 nm
Alternative Name Akt
Background Phospho AKT antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Synonyms: phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
Gene ID 207, 62241011
UniProt P31749
Application Notes Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
Protocol This monoclonal antibody is suitable for ELISA,immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffinembedded sections. No pre-treatment of sample is required. ELISA 1:20,000; WESTERN BLOT 1:500 - 1:3,000; IMMUNOHISTOCHEMISTRY 20 μg/ml; OTHER APPLICATIONS User Optimized
Restrictions For Research Use only
Format Liquid
Concentration 1.02 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody Immunohistochemistry of Mouse anti-AKT pS473 antibody. Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear and occasionally cytoplasmic. Staining: AKT pS473 as precipitated red signal with hematoxylin purple nuclear counterstain.
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (2) Immunohistochemistry using Anti-AKT pS473 monoclonal antibody shows detection of phosphory-lated AKT pS473 in human prostate tissue. The antibody was used at 20 ug/mL. The staining is much stronger than the weak basal level of phosphorylation in normal prostate tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. Signal was developed using Dako's Techmate streptavidin-biotin reagents. Personal communication, Glenna Burmer, Lifespan Biosciences, Seattle, WA.
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (3) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 3)
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (4) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 4)
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (5) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 5)
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (6) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 6)
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (7) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 7)
Product cited in: Ito, Nishibori, Ito et al.: "mTORC1 activation triggers the unfolded protein response in podocytes and leads to nephrotic syndrome." in: Laboratory investigation; a journal of technical methods and pathology, 2011 (PubMed).

General Jones, Jakubowicz, Pitossi et al.: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991 (PubMed).

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987 (PubMed).

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001 (PubMed).

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001 (PubMed).

Validation Images
Did you look for something else?
back to top