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Cofilin 1 (Non-Muscle) (CFL1) (pSer156), (pTyr140) antibody

Details for Product No. ABIN350239, Supplier: Log in to see
Antigen
  • cfl1
  • xac1
  • xac2
  • cfl
  • cb86
  • sb:cb86
  • zgc:56501
  • wu:fb18a04
  • wu:fk77b03
  • 12/8
  • ADF
  • ADF/cofilin
  • Adf
  • CG4254
  • Cadf
  • D-cof
  • D61
  • Dmel\\CG4254
  • T8
  • Tsr
  • cofilin
  • cofilin/ADF
  • cofilin/actin depolymerizing factor homolog
  • l(2)03101
  • l(2)k03101
  • l(2)k05633
  • l(2)k13409
  • ntf
  • CFL
  • AA959946
  • Cof
  • CFL1
Epitope
pTyr140, pSer156
51
20
19
14
13
12
12
10
8
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus), Human
249
131
118
7
7
4
4
2
2
1
1
Host
Rabbit
214
32
3
Clonality
Polyclonal
Conjugate
Un-conjugated
8
7
7
5
4
4
4
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (IHC), Western Blotting (WB)
163
92
75
43
37
34
18
14
8
4
3
2
2
1
Supplier
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Immunogen A synthetic peptide from the phospho Y140 & S156 of human Cofilin-1 (Cofilin, non-muscle isoform) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse and monkey.
Isotype IgG
Specificity Specific for Cofilin-1.
Alternative Name Cofilin 1 (CFL1 Antibody Abstract)
Background Function: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Subcellular location: Nucleus matrix. Cytoplasm, cytoskeleton. Note: Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
Tissue specificity: Widely distributed in various tissues. Also known as: Cofilin, non-muscle isoform.
Research Area Cytoskeleton
Pathways
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user. This antibody may also recognice the unphosphorylated form. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. ..." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Nakano, Kanai-Azuma, Kanai et al.: "Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family." in: Experimental cell research, Vol. 287, Issue 2, pp. 219-27, 2003 (PubMed).

Davidson, Haslam: "Dephosphorylation of cofilin in stimulated platelets: roles for a GTP-binding protein and Ca2+." in: The Biochemical journal, Vol. 301 ( Pt 1), pp. 41-7, 1994 (PubMed).

Ogawa, Tashima, Yumoto et al.: "Coding sequence of human placenta cofilin cDNA." in: Nucleic acids research, Vol. 18, Issue 23, pp. 7169, 1991 (PubMed).