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CDKN1B antibody (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1)) (C-Term)

Details for Product anti-CDKN1B Antibody No. ABIN350690, Supplier: Login to see
Antigen
  • CDKN1B
  • CIB
  • CIBP
  • KIP1
  • PRKDCIP
  • SIP2-28
  • p27Kip1
  • AA408329
  • AI843786
  • Kip1
  • p27
  • CDKN4
  • Cdki1b
  • P27KIP1
  • MEN1B
  • MEN4
  • cdkn1bl
  • cdkn1b
  • sb:cb611
  • wu:fb63g10
  • wu:fb64g10
  • wu:fe18e03
  • FAM14D
  • ISG12
  • ISG12A
  • P27
  • 1500011J20Rik
  • 2610202L11Rik
  • Bridge-1
Alternatives
anti-Human CDKN1B antibody for Immunohistochemistry (Frozen Sections)
Epitope
C-Term, AA 100-170
110
60
44
36
28
23
21
20
20
20
20
16
15
15
14
14
13
13
9
8
8
8
6
6
6
6
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus), Mouse (Murine)
590
202
144
21
21
16
10
7
6
5
3
2
2
2
2
1
1
Host
Rabbit
504
99
37
4
2
1
Clonality
Polyclonal
Conjugate
This CDKN1B antibody is un-conjugated
31
29
27
25
25
25
6
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
439
305
183
103
96
80
47
30
25
16
14
7
3
1
1
1
Supplier
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Immunogen A synthetic peptide from c-terminal region (aa 100-170) of human p27 Kip1 (KIP1, p27Kip1) conjugated to an immunogenic carrier protein was used as the immunogen.
Isotype IgG
Specificity Specific for p27Kip1.
Alternative Name p27 Kip1 (CDKN1B Antibody Abstract)
Background Function: Important regulator of cell cycle progrssion. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events. Decreased levels of p27Kip1, mainly due to proteosomal degradation, are found in various epithelial tumors originating from lung, breast, colon, ovary, esophagus, thyroid and prostate.
Subcellular location: Nucleus. Cytoplasm. Note: Nuclear and cytoplasmic in quiescent cells.
Tissue specificity: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney. Also known as: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, CDKN1B, KIP1, p27Kip1, p27 Kip 1.
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Grimmler, Wang, Mund et al.: "Cdk-inhibitory activity and stability of p27Kip1 are directly regulated by oncogenic tyrosine kinases." in: Cell, Vol. 128, Issue 2, pp. 269-80, 2007 (PubMed).

Viglietto, Motti, Bruni et al.: "Cytoplasmic relocalization and inhibition of the cyclin-dependent kinase inhibitor p27(Kip1) by PKB/Akt-mediated phosphorylation in breast cancer." in: Nature medicine, Vol. 8, Issue 10, pp. 1136-44, 2002 (PubMed).

Ishida, Kitagawa, Hatakeyama et al.: "Phosphorylation at serine 10, a major phosphorylation site of p27(Kip1), increases its protein stability." in: The Journal of biological chemistry, Vol. 275, Issue 33, pp. 25146-54, 2000 (PubMed).

Polyak, Lee, Erdjument-Bromage et al.: "Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals." in: Cell, Vol. 78, Issue 1, pp. 59-66, 1994 (PubMed).

Pietenpol, Bohlander, Sato et al.: "Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias." in: Cancer research, Vol. 55, Issue 6, pp. 1206-10, 1995 (PubMed).