TRKB antibody (Neurotrophic Tyrosine Kinase, Receptor, Type 2) (AA 400-450)

Details for Product anti-NTRK2 Antibody No. ABIN351077, Supplier: Log in to see
Antigen
  • trkb
  • trkb-b
  • xtrkb
  • si:ch211-220b11.1
  • trkB2
  • NTRK2
  • ntrk2
  • gp145-trkb
  • TRKB
  • GP145-TrkB
  • trk-B
  • AI848316
  • C030027L06Rik
  • Tkrb
  • trkB
  • RATTRKB1
  • TRKB1
  • Trk-B
  • TrkB
  • neurotrophic tyrosine kinase, receptor, type 2
  • neurotrophic tyrosine kinase, receptor, type 2b
  • NTRK2
  • ntrk2-b
  • ntrk2b
  • ntrk2-a
  • ntrk2
  • Ntrk2
Alternatives
anti-Human TRKB antibody for Cell Culture
Epitope
AA 400-450
25
25
18
16
16
15
14
14
14
11
10
9
9
8
8
8
6
6
6
5
5
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
313
195
180
14
13
10
8
8
4
2
1
1
1
1
Host
Rabbit
246
82
15
6
4
1
Clonality
Polyclonal
Conjugate
This TRKB antibody is un-conjugated
7
7
7
7
6
6
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
241
138
128
72
51
42
27
11
9
5
4
2
2
1
1
1
1
1
1
1
Options
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Immunogen A synthetic peptide from aa region 400-450 of human TrkB conjugated to an immunogenic carrier protein was used as the antigen.
Isotype IgG
Specificity Specific for TrkB.
Alternative Name TrkB (NTRK2 Antibody Abstract)
Background Function: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 and ARMS.
Subcellular location: Membrane, Single-pass type I membrane protein. Also known as: Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB/GP95-TrkB, Trk-B, BDNF/NT-3 growth factors receptor.
Research Area Cancer
Pathways RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Greenman, Stephens, Smith, Dalgliesh, Hunter, Bignell, Davies, Teague, Butler, Stevens, Edkins, OMeara, Vastrik, Schmidt, Avis, Barthorpe, Bhamra, Buck, Choudhury, Clements, Cole, Dicks, Forbes, Gray et al.: "Patterns of somatic mutation in human cancer genomes. ..." in: Nature, Vol. 446, Issue 7132, pp. 153-8, 2007 (PubMed).

Stoilov, Castren, Stamm: "Analysis of the human TrkB gene genomic organization reveals novel TrkB isoforms, unusual gene length, and splicing mechanism." in: Biochemical and biophysical research communications, Vol. 290, Issue 3, pp. 1054-65, 2002 (PubMed).

Ultsch, Wiesmann, Simmons, Henrich, Yang, Reilly, Bass, de Vos: "Crystal structures of the neurotrophin-binding domain of TrkA, TrkB and TrkC." in: Journal of molecular biology, Vol. 290, Issue 1, pp. 149-59, 1999 (PubMed).

Haniu, Talvenheimo, Le, Katta, Welcher, Rohde: "Extracellular domain of neurotrophin receptor trkB: disulfide structure, N-glycosylation sites, and ligand binding." in: Archives of biochemistry and biophysics, Vol. 322, Issue 1, pp. 256-64, 1995 (PubMed).

Nakagawara, Liu, Ikegaki, White, Yamashiro, Nycum, Biegel, Brodeur: "Cloning and chromosomal localization of the human TRK-B tyrosine kinase receptor gene (NTRK2)." in: Genomics, Vol. 25, Issue 2, pp. 538-46, 1995 (PubMed).

Shelton, Sutherland, Gripp, Camerato, Armanini, Phillips, Carroll, Spencer, Levinson: "Human trks: molecular cloning, tissue distribution, and expression of extracellular domain immunoadhesins." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 1 Pt 2, pp. 477-91, 1995 (PubMed).

Allen, Dawbarn, Eckford, Wilcock, Ashcroft, Colebrook, Feeney, MacGowan: "Cloning of a non-catalytic form of human trkB and distribution of messenger RNA for trkB in human brain." in: Neuroscience, Vol. 60, Issue 3, pp. 825-34, 1994 (PubMed).