TRKB (NTRK2)
(Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))
Binding Specificity
AA 730-821, pTyr816
Reactivity
Rat
Host
Sheep
Clonality
Polyclonal
Conjugate
This TRKB antibody is un-conjugated
Application
Western Blotting (WB), Immunohistochemistry (IHC)
Specificity
Specific for TrkB.
Cross-Reactivity
Human, Mouse, Rat
Cross-Reactivity (Details)
Other species not yet tested.
Purification
IgG
Immunogen
A synthetic peptide from AA 730-821 (including phospho Y816 site) of rat TrkB conjugated to ablue carrier protein was used as the antigen. The peptide is homologous in human and mouse.
NTRK2
Reactivity: Human
WB, PLA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. This antibody is also likely to recognise the unphosphorylated form.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in 500 μL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice
Avoid freeze and thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months
Target
TRKB (NTRK2)
(Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2))
FUNCTION: Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 and ARMS. SUBCELLULAR LOCATION: Membrane, Single-pass type I membrane protein.,Neurotrophic Receptors,Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, GP145-TrkB/GP95-TrkB, Trk-B, BDNF/NT-3 growth factors receptor