Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F) (1st Cytoplasmic Loop) antibody

Antigen: Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F)

Synonyms: RPC6, RPC39, MGC13517, MGC11656, 2810411G20Rik, 3010019O03Rik, 3110032A07Rik, MGC134486

Binding Site: 1st Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus), Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351161

Additional Information

Alternative name: RPC6

UniProt: Q61143

Immunogen: A synthetic peptide from the 1t cytoplasmic loop of rat TRPC6 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous with the corresponding sequence in mouse.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Also known as: Short transient receptor potential channel 6, TrpC6, Calcium entry channel, Trp6, transient receptor potential cation channel, subfamily C, member 6, Trrp6, mtrp6, AV025995, LLHWJM002, LLHWJM003, LLHWJM004.

Specificity: Appears to be specific for TRPC6.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Zhu, Jiang, Peyton et al.: "trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry." in: Cell, Vol. 85, Issue 5, pp. 661-71, 1996 (PubMed).

Boulay, Zhu, Peyton et al.: "Cloning and expression of a novel mammalian homolog of Drosophila transient receptor potential (Trp) involved in calcium entry secondary to activation of receptors coupled by the Gq class of G protein." in: The Journal of biological chemistry, Vol. 272, Issue 47, pp. 29672-80, 1997 (PubMed).

Buess, Engler, Hirsch et al.: "Search for oncogenic regulators in an autocrine tumor model using differential display PCR: identification of novel candidate genes including the calcium channel mtrp6." in: Oncogene, Vol. 18, Issue 7, pp. 1487-94, 1999 (PubMed).

Ramsey, Delling, Clapham: "An introduction to TRP channels." in: Annual review of physiology, Vol. 68, pp. 619-47, 2006 (PubMed).

Weissmann, Dietrich, Fuchs et al.: "Classical transient receptor potential channel 6 (TRPC6) is essential for hypoxic pulmonary vasoconstriction and alveolar gas exchange." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 50, pp. 19093-8, 2006 (PubMed).

Dietrich, Gudermann: "TRPC6." in: Handbook of experimental pharmacology, Issue 179, pp. 125-41, 2007 (PubMed).