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Methyl-CpG Binding Domain Protein 3 (MBD3) (C-Term) antibody

Details for Product No. ABIN356576, Supplier: Log in to see
Antigen
  • xmbd3
  • MGC69548
  • MBD3
  • DKFZp459N1635
  • AI181826
  • AU019209
Epitope
C-Term
22
17
16
6
4
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
82
23
9
2
2
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
63
18
2
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
72
34
10
10
7
5
2
Supplier
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding E279 of human MBD3.
Isotype Ig
Specificity This antibody is specific to MBD3 (C-term).
Purification Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name MBD3 (MBD3 Antibody Abstract)
Background DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).Synonyms: Methyl-CpG-binding domain protein 3
Gene ID 53615, 9606
UniProt O95983
Research Area Chromatin and Nuclear Signaling
Pathways
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (C-Term) antibody (ABIN356576) anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (C-Term) antibody
 image for anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (C-Term) antibody (ABIN356576) anti-Methyl-CpG Binding Domain Protein 3 (MBD3) (C-Term) antibody (Image 2)
Background publications Bader, Walker, Harrison: "Most microsatellite unstable sporadic colorectal carcinomas carry MBD4 mutations." in: British journal of cancer, Vol. 83, Issue 12, pp. 1646-9, 2001 (PubMed).

Petronzelli, Riccio, Markham et al.: "Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase." in: The Journal of biological chemistry, Vol. 275, Issue 42, pp. 32422-9, 2000 (PubMed).

Bhattacharya, Ramchandani, Cervoni et al.: "A mammalian protein with specific demethylase activity for mCpG DNA." in: Nature, Vol. 397, Issue 6720, pp. 579-83, 1999 (PubMed).

Hendrich, Bird: "Identification and characterization of a family of mammalian methyl-CpG binding proteins." in: Molecular and cellular biology, Vol. 18, Issue 11, pp. 6538-47, 1998 (PubMed).