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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer129) antibody

Details for Product No. ABIN358075
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Antigen
Synonyms
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AK ... show more
AKT, AKT1, pkb, xAct, v-akt, v-akt1, akt-1, AKT/PKB, Akt, Akt/PKB, Akt1, PKB, CG4006, D-Akt, DAKT1, DAKT1/PKB, DAkt, DAkt1, DPKB, DRAC-PK, DRAC-PK66, DRAC-PK85, Dakt, Dakt1, Dmel\\CG4006, Dpkb, PKB/AKT, PKB/Akt, PKB/dAKT, RAC, RacPK, akt, akt1, dAKT, dAKT/dPKB, dAKT1, dAkt, dAkt/PKB, dAkt1, dPKB, dakt, dakt1, l(3)04226, l(3)89Bq, p-Akt, pAkt, ATAKT1, F18A8.2, F18A8_2, K+ transporter 1, POTASSIUM TRANSPORTER, Rac, PKBalpha, CWS6, PKB-ALPHA, PRKBA, RAC-ALPHA show less
Epitope
pSer129
(133), (87), (64), (60), (58), (47), (35), (33), (29), (19), (18), (17), (17), (17), (16), (14), (10), (10), (9), (8), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(932), (553), (523), (164), (153), (115), (94), (24), (18), (4), (2), (1), (1), (1), (1)
Host
Rabbit
(795), (144), (10), (5), (3), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(33), (27), (26), (22), (14), (13), (13), (13), (13), (13), (13), (13), (13), (13), (7), (7), (7), (7), (1), (1), (1), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(714), (426), (297), (158), (149), (133), (119), (65), (58), (41), (11), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.4 mL
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Catalog No. ABIN358075
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S129 of human AKT1.
Specificity This antibody detects AKT1/PKB pSer129. Predicted to cross react with Mouse (100% Antigen Homology).
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name AKT1 / PKB
Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Alternate names: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Molecular Weight 55686 Da
Gene ID 207
NCBI Accession 9606
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer129) antibody anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer129) antibody
anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer129) antibody (2) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer129) antibody (Image 2)
Background publications Bellacosa, Chan, Ahmed et al.: "Akt activation by growth factors is a multiple-step process: the role of the PH domain." in: Oncogene, Vol. 17, Issue 3, pp. 313-25, 1998 (PubMed).

Margolskee: "Molecular mechanisms of bitter and sweet taste transduction." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 1-4, 2002 (PubMed).

McCulloch, Omens: "Myocyte shearing, myocardial sheets, and microtubules." in: Circulation research, Vol. 98, Issue 1, pp. 1-3, 2006 (PubMed).

Dephoure, Zhou, Villén et al.: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008 (PubMed).

Validation Images
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