V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (Ser43), (pSer43) antibody

Details for Product No. ABIN358300
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Antigen
Synonyms c-raf, craf, raf1, RAF1, raf, CRAF, NS5, Raf-1, c-Raf, 6430402F14Rik, AA990557, BB129353, Craf1, D830050J10Rik, v-Raf, AFR1, RAF, Afr-1, Afr1, Raf, mKIAA0854
Epitope
Ser43, pSer43
(67), (48), (32), (31), (26), (26), (25), (24), (24), (22), (21), (18), (17), (17), (17), (17), (15), (14), (12), (12), (8), (8), (7), (7), (7), (6), (6), (6), (6), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(638), (352), (348), (151), (149), (120), (113), (12), (5), (2), (2), (1), (1), (1)
Host
Rabbit
(599), (34), (15), (8), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(28), (28), (28), (15), (15), (15), (13), (13), (13), (13), (13), (13), (13), (13)
Application
ELISA
(415), (287), (158), (130), (102), (100), (32), (16), (15), (12), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.4 mL
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Catalog No. ABIN358300
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser43 of human RAF1.
Specificity This antibody detects RAF1 pSer43. Predicted to cross react with Mouse (100% Antigen Homology).
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RAF1
Background Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
Alternate names: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Molecular Weight 72921 Da
Gene ID 5894, 9606
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Application Notes ELISA: 1/1,000. Dot Blot: 1/100-1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (Ser43), (pSer43) antibody anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (Ser43), (pSer43) antibody
Background publications Goetz, Harmon, ONeil et al.: "STAT5 activation underlies IL7 receptor-dependent B cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 8, pp. 4770-8, 2004 (PubMed).

Rashdan, Fogel, McHenry et al.: "Improved stent characteristics for prophylaxis of post-ERCP pancreatitis." in: Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association, Vol. 2, Issue 4, pp. 322-9, 2004 (PubMed).

Akula, Ford, Whitman et al.: "Raf promotes human herpesvirus-8 (HHV-8/KSHV) infection." in: Oncogene, Vol. 23, Issue 30, pp. 5227-41, 2004 (PubMed).

Ku, Fu, Omary: "Raf-1 activation disrupts its binding to keratins during cell stress." in: The Journal of cell biology, Vol. 166, Issue 4, pp. 479-85, 2004 (PubMed).

Dasgupta, Sun, Wang et al.: "Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9527-41, 2004 (PubMed).

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