RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer259)

Details for Product anti-RAF1 Antibody No. ABIN358378, Supplier: Log in to see
Antigen
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Alternatives
anti-Human RAF1 antibody for Cytometry by Time of Flight
Epitope
pSer259
73
70
47
39
38
37
28
28
28
27
22
22
22
20
19
18
17
15
15
15
15
15
14
14
10
8
8
7
6
6
6
6
6
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
797
411
386
25
23
16
16
13
8
8
8
5
4
4
2
2
1
1
1
Host
Rabbit
760
44
12
6
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
44
44
43
29
29
29
13
13
12
12
12
12
12
12
12
12
12
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
481
343
202
172
156
95
49
33
19
17
17
7
2
2
2
1
Options
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser259 of human RAF1.
Isotype Ig
Specificity This antibody detects RAF1 pSer259.
Purification Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
Molecular Weight 73052 Da
Gene ID 5894, 9606
UniProt P04049
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1) (pSer259) (ABIN358378) anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer259) antibody
Background publications Rashdan, Fogel, McHenry, Sherman, Temkit, Lehman: "Improved stent characteristics for prophylaxis of post-ERCP pancreatitis." in: Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association, Vol. 2, Issue 4, pp. 322-9, 2004 (PubMed).

Dasgupta, Sun, Wang, Fusaro, Betts, Padmanabhan, Sebti, Chellappan: "Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9527-41, 2004 (PubMed).

Ku, Fu, Omary: "Raf-1 activation disrupts its binding to keratins during cell stress." in: The Journal of cell biology, Vol. 166, Issue 4, pp. 479-85, 2004 (PubMed).

Akula, Ford, Whitman, Hamden, Shelton, McCubrey: "Raf promotes human herpesvirus-8 (HHV-8/KSHV) infection." in: Oncogene, Vol. 23, Issue 30, pp. 5227-41, 2004 (PubMed).

Goetz, Harmon, ONeil, Burchill, Farrar: "STAT5 activation underlies IL7 receptor-dependent B cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 8, pp. 4770-8, 2004 (PubMed).