DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) (C-Term) antibody

Details for Product No. ABIN359623
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Antigen
Synonyms wu:fa56a07, wu:fb11e01, wu:fb16c10, wu:fb53b05, G17P1, HLR1, HUMP68, p68, 2600009A06Rik, Hlr1
Epitope
C-Term
(18), (16), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(79), (45), (44), (26), (14), (7)
Host
Rabbit
(63), (10), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
ELISA, Western Blotting (WB)
(47), (26), (20), (19), (18), (18), (9), (7), (2), (2)
Pubmed 2 references available
Quantity 0.4 mL
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Catalog No. ABIN359623
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DDX5.
Specificity This antibody reacts to DDX5.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Alternative Name DDX5
Background DDX5 is putative RNA helicases. The protein is implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This protein is a RNA- dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen.
Alternate names: DDX-5, DEAD box protein 5, G17P1, HELR, HLR1, Probable ATP-dependent RNA helicase DDX5, RNA helicase p68
Gene ID 1655, 9606
UniProt P17844
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Background publications Ong, Mittler, Mann: "Identifying and quantifying in vivo methylation sites by heavy methyl SILAC." in: Nature methods, Vol. 1, Issue 2, pp. 119-26, 2005 (PubMed).

Daub, Olsen, Bairlein et al.: "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." in: Molecular cell, Vol. 31, Issue 3, pp. 438-48, 2008 (PubMed).

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