PRKAR1A
Reactivity: Human
WB, ELISA
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target
PRKAR1A
(Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))
ACRDYS1 antibody, ADOHR antibody, CAR antibody, CNC antibody, CNC1 antibody, PKR1 antibody, PPNAD1 antibody, PRKAR1 antibody, TSE1 antibody, 1300018C22Rik antibody, RIalpha antibody, Tse-1 antibody, Tse1 antibody, RIIA antibody, prkar1a antibody, zgc:92515 antibody, im:7047729 antibody, cnc1 antibody, pkr1 antibody, ppnad1 antibody, prkar1 antibody, tse1 antibody, protein kinase cAMP-dependent type I regulatory subunit alpha antibody, protein kinase, cAMP dependent regulatory, type I, alpha antibody, protein kinase cAMP-dependent type 1 regulatory subunit alpha antibody, protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a antibody, cAMP-dependent protein kinase type I-alpha regulatory subunit pseudogene antibody, protein kinase cAMP-dependent type I regulatory subunit alpha S homeolog antibody, PRKAR1A antibody, Prkar1a antibody, prkar1aa antibody, LOC707275 antibody, prkar1a.S antibody
Background
CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKR1 is one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Functional null mutations in the gene cause Carney complex (CNC), an autosomal dominant multiple neoplasia syndrome. The gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2.Synonyms: CAR, CNC1, PKR1, PRKAR1, TSE1, Tissue-specific extinguisher 1, cAMP-dependent protein kinase type I-alpha regulatory subunit