Glucokinase (Hexokinase 4) Regulator (GCKR) antibody

Antigen: Glucokinase (Hexokinase 4) Regulator (GCKR)

Synonyms: GKRP, GLRE, MGC93611, MGC19300, gckr

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN361187

Additional Information

Alternative name: GCKR

Gene ID: 2646, http://www.ncbi.nlm.nih.gov/sites/entrez?deb=gene&term= 231103

Immunogen: Peptide with sequence KRFQHVIETPEPGK -C, from the N-Terminus of the protein sequence according to NP_001477.1

Description: All names and symbols: GCKR, glucokinase (hexokinase 4) regulator, GKRP, glucokinase (hexokinase 4) regulatory protein, glucokinase regulator, glucokinase regulatory protein, hexokinase 4. Official Symbol: GCKR. RefSeq number(s): NP_001477.1.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, N-Terminus

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 70kDa band observed in Mouse and Rat Liver lysates (calculated MW of 68.7kDa according to Human NP_001477.1 and of 68.9kDa according to Rat NP_037252.1). Recommended concentration: 0.1-0.3µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Atherosclerosis, Metabolism

Restrictions: For Research Use only

Images

EB09275 (0.1µg/ml) staining of Rat Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.