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Activating Transcription Factor 2 (ATF2) (pSer490), (pSer498) antibody

Details for Product No. ABIN361474
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Antigen
Synonyms CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420, CRE-BP1, CREB2, HB16, TREB7, Atf-2, CRE-BP, Creb2, D130078H02Rik, D18875, Tg(Gzma-Klra1)7Wum, mXBP, atf2-A, atf2l, ATF1, ATF2, HFB1-GDNF, HSCR3
Epitope
pSer490, pSer498
(67), (60), (52), (48), (34), (32), (31), (31), (24), (24), (23), (21), (20), (18), (18), (15), (14), (8), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(534), (363), (347), (111), (89), (72), (62), (12), (2), (2), (1), (1)
Host
Rabbit
(498), (23), (8), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(18), (18), (18), (16), (16), (16), (16), (16), (16), (16), (16), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
(323), (189), (160), (144), (110), (106), (51), (16), (12), (10), (5), (3), (3), (2), (1), (1)
Pubmed 3 references available
Quantity 100 µL
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Catalog No. ABIN361474
379.50 $
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Specificity Specific for ~74k ATF2 protein phosphorylated at Ser490,498. The antibody also recognizes the phosphorylated ~54k splice form of ATF2.
Purification Affinity-purified
Alternative Name ATF2
Background The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (Maekawa et al., 1989). ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2 (Ivanov et al., 2003, Morton et al., 2004). Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF (Fuchs et al., 2000), Morton et al., 2004). Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation (Karin and Hunter, 1995). ATF expression has also been linked to the depression in humans (Laifenfeld et al., 2004). Anti-Phospho Ser490,498 ATF2 Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immuno-labeling of the ~74k ATF2 protein phosphorylated at Ser490 and Ser498.
Application Notes Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 Quality Control: Western blots performed on each lot.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Supplier Images
anti-Activating Transcription Factor 2 (ATF2) (pSer490), (pSer498) antibody Western blots of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immuno-labeling of the ~74k ATF2 protein phosphorylated at Ser490 and Ser498.
Product cited in: Chappell, Whalen, Gurai et al.: "Analysis of Flow Cytometry DNA Damage Response Protein Activation Kinetics after Exposure to X Rays and High-Energy Iron Nuclei." in: Radiation research, 2010 (PubMed). (1:1000).

Wang, Saha, Hada et al.: "Novel Smad proteins localize to IR-induced double-strand breaks: interplay between TGFβ and ATM pathways." in: Nucleic acids research, Vol. 41, Issue 2, pp. 933-42, 2013 (PubMed).

Muñoz, Kawahara, Yannone: "An autonomous chromatin/DNA-PK mechanism for localized DNA damage signaling in mammalian cells." in: Nucleic acids research, 2013 (PubMed).

Validation Images
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