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ATF2 antibody (Activating Transcription Factor 2) (pSer490)

Details for Product anti-ATF2 Antibody No. ABIN361474, Supplier: Log in to see
Antigen
  • Atf-2
  • ATF1
  • ATF2
  • atf2-A
  • atf2l
  • CG3749
  • CG12850
  • CG30420
  • CHN1
  • CRE-BP
  • CRE-BP1
  • Creb2
  • CREB2
  • D18875
  • D130078H02Rik
  • dATF-2
  • DmelCG30420
  • HB16
  • HFB1-GDNF
  • HSCR3
  • mXBP
  • Tg(Gzma-Klra1)7Wum
  • TREB7
Alternatives
anti-Human ATF2 antibody for Western Blotting
Epitope
pSer490, pSer498
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40
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Reactivity
Human
566
323
294
20
9
6
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Host
Rabbit
518
32
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Clonality
Polyclonal
Conjugate
This ATF2 antibody is un-conjugated
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9
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6
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Supplier
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Specificity Specific for ~74k ATF2 protein phosphorylated at Ser490,498. The antibody also recognizes the phosphorylated ~54k splice form of ATF2.
Purification Affinity-purified
Alternative Name ATF2 (ATF2 Antibody Abstract)
Background The activating transcription factor ATF2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (Maekawa et al., 1989). ATF2 has been implicated in the transcriptional regulation of a number of genes including cytokines, cell cycle control and apoptosis. Various forms of cellular stress, including inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF2 (Ivanov et al., 2003, Morton et al., 2004). Stress induced ATF-dependent transcription is dependent on phosphorylation of ATF (Fuchs et al., 2000), Morton et al., 2004). Serine 490 and serine 498 are novel phosphorylation sites on ATF that have recently been identified. ATF2 is particularly abundant in the brain and the ATF2 family of transcription factors is considered an important substrate of signals upstream of the activation of genes associated with neuronal growth and differentiation (Karin and Hunter, 1995). ATF expression has also been linked to the depression in humans (Laifenfeld et al., 2004). Anti-Phospho Ser490,498 ATF2 Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immuno-labeling of the ~74k ATF2 protein phosphorylated at Ser490 and Ser498.
Pathways MAPK Signaling, RTK Signaling, TCR Signaling
Application Notes Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 Quality Control: Western blots performed on each lot.
Restrictions For Research Use only
Format Liquid
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-ATF2 antibody (Activating Transcription Factor 2) (pSer490) (ABIN361474) Western blots of human melanoma cells incubated with varying doses of the radiomimeti...
Product cited in: Muñoz, Kawahara, Yannone: "An autonomous chromatin/DNA-PK mechanism for localized DNA damage signaling in mammalian cells." in: Nucleic acids research, Vol. 41, Issue 5, pp. 2894-906, 2013

Wang, Saha, Hada, Anderson, Pluth, ONeill, Cucinotta: "Novel Smad proteins localize to IR-induced double-strand breaks: interplay between TGFβ and ATM pathways." in: Nucleic acids research, Vol. 41, Issue 2, pp. 933-42, 2013

Chappell, Whalen, Gurai, Ponomarev, Cucinotta, Pluth: "Analysis of Flow Cytometry DNA Damage Response Protein Activation Kinetics after Exposure to X Rays and High-Energy Iron Nuclei." in: Radiation research, 2010 (1:1000).

Background publications Bhoumik, Takahashi, Breitweiser, Shiloh, Jones, Ronai: "ATM-dependent phosphorylation of ATF2 is required for the DNA damage response." in: Molecular cell, Vol. 18, Issue 5, pp. 577-87, 2005

Laifenfeld, Karry, Grauer, Klein, Ben-Shachar: "ATF2, a member of the CREB/ATF family of transcription factors, in chronic stress and consequent to antidepressant treatment: animal models and human post-mortem brains." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 29, Issue 3, pp. 589-97, 2004

Morton, Davis, Cohen: "Signalling pathways involved in multisite phosphorylation of the transcription factor ATF-2." in: FEBS letters, Vol. 572, Issue 1-3, pp. 177-83, 2004

Ivanov, Bhoumik, Ronai: "Death receptors and melanoma resistance to apoptosis." in: Oncogene, Vol. 22, Issue 20, pp. 3152-61, 2003

Fuchs, Tappin, Ronai: "Stability of the ATF2 transcription factor is regulated by phosphorylation and dephosphorylation." in: The Journal of biological chemistry, Vol. 275, Issue 17, pp. 12560-4, 2000

Karin, Hunter: "Transcriptional control by protein phosphorylation: signal transmission from the cell surface to the nucleus." in: Current biology : CB, Vol. 5, Issue 7, pp. 747-57, 1995

Maekawa, Sakura, Kanei-Ishii, Sudo, Yoshimura, Fujisawa, Yoshida, Ishii: "Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain." in: The EMBO journal, Vol. 8, Issue 7, pp. 2023-8, 1989