You are viewing an incomplete version of our website. Please click to reload the website as full version.

LAMP1 antibody (Lysosomal-Associated Membrane Protein 1)

Details for Product anti-LAMP1 Antibody No. ABIN361796, Supplier: Log in to see
Antigen
Reactivity
Hamster, Human, Mouse (Murine), Rat (Rattus)
258
123
82
24
14
10
5
3
2
Host
Mouse
190
105
57
2
Clonality (Clone)
Monoclonal ()
Conjugate
This LAMP1 antibody is un-conjugated
32
26
23
13
7
6
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
179
168
76
74
73
64
46
33
27
19
14
12
4
3
3
2
2
2
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen Rat liver lysosomal membrane preparations
Clone Ly1C6
Specificity Detects ~120 kDa.
Sensitivity 1 µg/ml was sufficient for detection of LAMP1 in rat liver miscrosome by ECL immunoblot analysis.
Purification Protein G Purified
Alternative Name LAMP1 (LAMP1 Antibody Abstract)
Background Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl¬terminal tyrosine ba based motif (YXXI) (1). If there is a disturbance in this spacing, lysosome localization of LAMP1 is abolished and the mutant protein then cycles between the membrane and the endosome (3). This stimulation has also been shown to have an associated release of histamine, leukotriene C (4) and prostaglandin D (2), which shows that LAMP-1 and LAMP-2 are activation markers for normal mast cells (1). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (4).
Cellular Localization: Cell Membrane | Endosome | Lysosome | Endosome membrane | Lysosome membrane
Gene ID 25328
NCBI Accession NP_036989
UniProt P14562
Research Area Cancer, Hematopoietic Progenitors, Organelles
Application Notes Recommended Dilution: WB (1:1000), ICC/IF (1:1000), optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-LAMP1 antibody (Lysosomal-Associated Membrane Protein 1) (ABIN361796) Lamp1 (Ly1C6), IF in transfected HeLa cells Courtesy of Robert H Edwards, U of Cali,...
Western Blotting (WB) image for anti-LAMP1 antibody (Lysosomal-Associated Membrane Protein 1) (ABIN361796) LAMP1 WB 1 in 1000 rat liver micosomes 20ug copy.
Product cited in: Harada, Hiasa: "Immunological identification of vesicular nucleotide transporter in intestinal L cells." in: Biological & pharmaceutical bulletin, Vol. 37, Issue 7, pp. 1090-5, 2014 (PubMed).

Hiasa, Togawa, Miyaji et al.: "Essential role of vesicular nucleotide transporter in vesicular storage and release of nucleotides in platelets." in: Physiological reports, Vol. 2, Issue 6, 2014 (PubMed).

Background publications Grützkau, Smorodchenko, Lippert et al.: "LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 61, Issue 1, pp. 62-8, 2004 (PubMed).

Jones, Jiang, Yamamoto et al.: "Tuberin is a component of lipid rafts and mediates caveolin-1 localization: role of TSC2 in post-Golgi transport." in: Experimental cell research, Vol. 295, Issue 2, pp. 512-24, 2004 (PubMed).

Kannan, Stewart, Bounds et al.: "Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion ..." in: Cellular immunology, Vol. 171, Issue 1, pp. 10-9, 1996 (PubMed).

Rohrer, Schweizer, Russell et al.: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, 1996 (PubMed).

Lewis, Green, Marsh et al.: "Glycoproteins of the lysosomal membrane." in: The Journal of cell biology, Vol. 100, Issue 6, pp. 1839-47, 1985 (PubMed).