»
Antibodies
»
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer21) antibody
Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer21) antibody
| Antigen |
|
| Synonyms |
GSK3A, 2700086H06Rik, MGC159759, DKFZp686D0638, CG5182, DmelCG5182, GSK3a, GSK3, GSK-3[a], GSK-3[b], xgsk3 |
|
Epitope
|
|
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
Human (64), Mouse (Murine) (46), Rat (Rattus) (40), Cow (Bovine) (6), Dog (Canine) (5), Chicken (2), Monkey (2), Pig (Porcine) (2), Rabbit (2), Hamster (1), Horse (Equine) (1), Sheep (Ovine) (1), Zebrafish (Brachydanio rerio) (1)
|
|
Application
|
 Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
|
| Catalog no. |
ABIN361885 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
280.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Alternative name
|
GSK3alpha (Phospho-Ser21)
|
|
Swiss-Prot
|
P49840
|
|
Immunogen
|
The antiserum was produced against synthesized phosphopeptide derived from humanGSK3alpha around the phosphorylation site of serine 21 (T-S-S P -F-A).
|
|
Isotype
|
IgG (Matching secondary antibodies)
|
|
Specificity
|
GSK3alpha (Phospho-Ser21) (Human) antibody detects endogenous levels of GSK3alpha only whenphosphorylated at serine 21and does not detect GSK-3beta when phosphorylated at Ser9.
|
|
Application Notes
|
WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
|
|
Concentration
|
50µg/50µl
|
|
Purification
|
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
|
|
Buffer
|
Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
|
|
Storage
|
Store at -20°C. Avoid repeat freeze-thaw cycles.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Glycogen Synthase Kinase 3 alpha (GSK3a)", type "Antibodies"
|
Hosts
|
Rabbit (50), Mouse (26)
|
|
Reactivities
|
Human (64), Mouse (Murine) (46), Rat (Rattus) (40), Cow (Bovine) (6), Dog (Canine) (5), Chicken (2), Monkey (2), Pig (Porcine) (2), Rabbit (2), Hamster (1), Horse (Equine) (1), Sheep (Ovine) (1), Zebrafish (Brachydanio rerio) (1)
|
|
Applications
|
Western Blotting (WB) (66), Immunofluorescence (IF) (22), Immunohistochemistry (IHC) (22), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Immunoprecipitation (IP) (15), Enzyme Immunoassay (EIA) (14), ELISA (10), Flow Cytometry (FACS) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunocytochemistry (ICC) (1)
|
|
Conjugates
|
HRP (4), Sepharose (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), PE,Cy5.5 (1)
|
|
Epitopes
|
pSer21 (15), C-Term (3), pS21 (2), S21 (1)
|
