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MAP2K2 antibody (Mitogen-Activated Protein Kinase Kinase 2) (pThr394)

Details for Product anti-MAP2K2 Antibody No. ABIN361888, Supplier: Log in to see
Antigen
  • AA589381
  • ATMKK2
  • CFC4
  • F27B13.50
  • F27B13_50
  • MAP KINASE KINASE 1
  • MAP kinase kinase 2
  • MAPKK2
  • MEK2
  • MK1
  • MK2
  • MKK2
  • PRKMK2
  • Prkmk2
Alternatives
anti-Human MAP2K2 antibody for Western Blotting
Epitope
pThr394
32
23
15
15
14
14
12
10
10
8
8
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
230
106
89
8
4
3
2
2
Host
Rabbit
189
38
9
1
Clonality
Polyclonal
Conjugate
This MAP2K2 antibody is un-conjugated
10
10
9
7
7
7
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
187
80
78
38
27
26
23
15
13
11
7
4
2
1
1
Supplier
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Immunogen Peptide sequence around phosphorylation site of pThr394 (P-G-T (p) -P-T) derived from Human MEK-2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name MEK-2 (MAP2K2 Antibody Abstract)
Background Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
Molecular Weight 44 kDa
NCBI Accession NP_109587
UniProt P36507
Research Area Cancer, Tyrosine Kinases, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Alessi, Saito, Campbell, Cohen, Sithanandam, Rapp, Ashworth, Marshall, Cowley: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, 1994

Rosen, Ginty, Weber, Greenberg: "Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras." in: Neuron, Vol. 12, Issue 6, pp. 1207-21, 1994

Cowley, Paterson, Kemp, Marshall: "Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells." in: Cell, Vol. 77, Issue 6, pp. 841-52, 1994

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992