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V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody
| Antigen | V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) |
| Synonyms |
AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, ...
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| Epitope |
 Alternatives pSer473 |
| Clonality | Polyclonal |
| Host |
Alternatives Rabbit |
| Reactivity |
Alternatives Human, Mouse (Murine), Rat (Rattus) |
| Application |
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
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| Catalog no. | ABIN361977 |
| Quantity | 50µg (50µg/50µl) (Variants) |
| Price | 280.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business days |
Additional Information
| Alternative name | Akt (Phospho-Ser473) |
| Swiss-Prot | P31749 |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human Aktaround the phosphorylation site of serine 473 (Q-F-S P -Y-S). |
| Isotype | IgG (Matching secondary antibodies) |
| Specificity | Akt (Phospho-Ser473) (Human) antibody detects endogenous levels of Akt only when phosphorylatedat serine 473. |
| Synonyms | AKT, PKB, RAC, PRKBA, MGC99656, PKB-ALPHA, RAC-ALPHA, Akt, pkb, xAct, akt-1, v-akt, v-akt1, AKT1, AKT/PKB, D-Akt, DAKT1, DAKT1/PKB, DPKB, DRAC-PK, DRAC-PK66; DRAC-PK85, Dakt, PKB/AKT, PKB/dAKT, RacPK, dAKT/dPKB, dAkt/PKB, l(3)04226, l(3)89Bq, DmelCG4006, CG4006 |
Application Details
| Application Notes | WB: 1:500~1:1000 IHC: 1:50~1:100 |
| Concentration | 50µg/50µl |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site. |
| Buffer | Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage | Store at -20°C. Avoid repeat freeze-thaw cycles. |
| Restrictions | For Research Use only |
Alternatives
Alternatives for antigen "V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)", type "Antibodies"
| Hosts | Rabbit (162), Mouse (60), Sheep (4), Chicken (1) |
| Reactivities | Human (214), Mouse (Murine) (121), Rat (Rattus) (112), Chicken (7), Dog (Canine) (4), Chimpanzee (2), Cow (Bovine) (2), Frog (1), Rabbit (1), Sheep (Ovine) (1) |
| Applications | Western Blotting (WB) (182), Immunohistochemistry (IHC) (63), Enzyme Immunoassay (EIA) (60), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (59), ELISA (54), Immunofluorescence (IF) (45), Immunoprecipitation (IP) (16), Immunohistochemistry (Frozen Sections) (IHC (fro)) (11), Dot Blot (Dot) (9), Flow Cytometry (FACS) (4), Immunocytochemistry (ICC) (2), ELISA (Detection) (1) |
| Conjugates | Biotin (2), APC (1) |
| Epitopes | pSer473 (20), pThr308 (14), pThr450 (5), pTyr315/316/312 (4), S129 (3), C-Term (2), N-Term (2), S246 (2), pS124 (2), pS473 (2), pThr308/309 (2), pY326 (2), N-Term G37 (1), S124 (1), S473 (1), pT308 (1) |
