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p53 antibody (Tumor Protein P53) (pSer37)

Details for Product anti-TP53 Antibody No. ABIN362053, Supplier: Log in to see
Antigen
Alternatives
anti-Human p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
pSer37
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86
62
54
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49
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9
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2
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2
2
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2
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2
2
2
2
2
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2
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2
2
1
1
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1
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1
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1
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1
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Reactivity
Human
1757
319
270
97
34
18
14
11
11
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10
9
5
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3
3
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2
2
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1
Host
Rabbit
1238
605
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
48
44
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27
20
20
17
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14
2
2
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2
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1
Application
Immunofluorescence (IF), Western Blotting (WB)
1370
575
526
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234
160
98
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53
39
35
17
16
11
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11
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Immunogen Peptide sequence around phosphorylation site of pSer37 (L-P-S (p) -Q-A) derived from Human p53. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of p53 only when phosphorylated at serine 37.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name p53 (TP53 Antibody Abstract)
Background P53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes
Molecular Weight 53 kDa
NCBI Accession NP_000537
UniProt P04637
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Guan, Li, Yang et al.: "Cell cycle arrest and apoptosis of OVCAR-3 and MCF-7 cells induced by co-immobilized TNF-? plus IFN-? on polystyrene and the role of p53 activation." in: Biomaterials, Vol. 33, Issue 26, pp. 6162-71, 2012 (PubMed).

Background publications Solomon, Pasupuleti, Xu et al.: "Inhibition of SIRT1 catalytic activity increases p53 acetylation but does not alter cell survival following DNA damage." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 28-38, 2005 (PubMed).

Cases, de Lorenzo: "The black cat/white cat principle of signal integration in bacterial promoters." in: The EMBO journal, Vol. 20, Issue 1-2, pp. 1-11, 2001 (PubMed).

Duboule: "Hox is in the hair: a break in colinearity?" in: Genes & development, Vol. 12, Issue 1, pp. 1-4, 1998 (PubMed).