You are viewing an incomplete version of our website. Please click to reload the website as full version.

Insulin Receptor Substrate 1 (IRS1) (pSer312) antibody

Details for Product No. ABIN362134, Supplier: Log in to see
Antigen
  • irs1
  • irsu
  • IRS1
  • HIRS-1
  • G972R
  • IRS-1
  • IRS1IRM
  • irs-1
  • irs1-a
  • insulin receptor substrate 2
  • insulin receptor substrate 1
  • irs2
  • IRS1
  • Irs1
  • irs1
Alternatives
anti-Human Insulin Receptor Substrate 1 antibody for Enzyme Immunoassay
Epitope
pSer312
59
52
46
46
28
25
22
19
17
17
17
16
15
15
15
15
15
12
10
9
8
6
6
5
5
5
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
565
456
433
19
16
6
5
4
4
1
1
Host
Rabbit
560
32
Clonality
Polyclonal
Conjugate
Un-conjugated
13
11
11
11
10
10
10
10
10
10
10
10
10
10
2
2
1
1
Application
Western Blotting (WB)
343
228
152
130
127
108
31
29
14
12
4
3
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen Peptide sequence around phosphorylation site of pSer312 (A-T-S (p) -P-A) derived from Human IRS-1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name IRS-1 (IRS1 Antibody Abstract)
Background May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit
Molecular Weight 180 kDa
NCBI Accession NP_005535
UniProt P35568
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis
Application Notes Western blotting: 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Tzatsos, Kandror: "Nutrients suppress phosphatidylinositol 3-kinase/Akt signaling via raptor-dependent mTOR-mediated insulin receptor substrate 1 phosphorylation." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 63-76, 2005 (PubMed).

Ozes, Akca, Mayo, Gustin, Maehama, Dixon, Donner: "A phosphatidylinositol 3-kinase/Akt/mTOR pathway mediates and PTEN antagonizes tumor necrosis factor inhibition of insulin signaling through insulin receptor substrate-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 8, pp. 4640-5, 2001 (PubMed).

Szanto, Kahn: "Selective interaction between leptin and insulin signaling pathways in a hepatic cell line." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 5, pp. 2355-60, 2000 (PubMed).