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ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr319)

Details for Product anti-ZAP70 Antibody No. ABIN362163, Supplier: Log in to see
Antigen
  • 70kDa
  • AI327364
  • MGC80670
  • MGC88962
  • mrtle
  • mur
  • SRK
  • Srk
  • STCD
  • STD
  • TZK
  • ZAP-70
  • zap70
  • ZAP70
Alternatives
anti-Human ZAP70 antibody for Western Blotting
Epitope
pTyr319
64
51
47
46
25
22
21
21
20
15
15
15
14
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
530
186
121
4
4
3
2
2
2
Host
Rabbit
298
212
15
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
31
24
18
16
10
8
8
7
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunofluorescence (IF), Western Blotting (WB)
296
162
154
148
120
68
54
44
24
7
5
5
4
4
3
2
Supplier
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Immunogen Peptide sequence around phosphorylation site of pTyr319 (S-P-Y (p) -S-D) derived from Human Zap-70. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of VEGFR2 only when phosphorylated at tyrosine 1214.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Zap-70 (ZAP70 Antibody Abstract)
Background Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Molecular Weight 70 kDa
NCBI Accession NP_001070
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Adjali, Marodon, Steinberg, Mongellaz, Thomas-Vaslin, Jacquet, Taylor, Klatzmann: "In vivo correction of ZAP-70 immunodeficiency by intrathymic gene transfer." in: The Journal of clinical investigation, Vol. 115, Issue 8, pp. 2287-95, 2005

Salomon, Ficarro, Brill, Brinker, Phung, Ericson, Sauer, Brock, Horn, Schultz, Peters: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003

Ku, Yablonski, Manser, Lim, Weiss: "A PAK1-PIX-PKL complex is activated by the T-cell receptor independent of Nck, Slp-76 and LAT." in: The EMBO journal, Vol. 20, Issue 3, pp. 457-65, 2001

Tang, Sawasdikosol, Chang, Burakoff: "SLAP, a dimeric adapter protein, plays a functional role in T cell receptor signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 17, pp. 9775-80, 1999

Williams, Irvin, Sutor, Chini, Yacyshyn, Bubeck Wardenburg, Dalton, Chan, Abraham: "Phosphorylation of Tyr319 in ZAP-70 is required for T-cell antigen receptor-dependent phospholipase C-gamma1 and Ras activation." in: The EMBO journal, Vol. 18, Issue 7, pp. 1832-44, 1999

Zhao, Weiss: "Enhancement of lymphocyte responsiveness by a gain-of-function mutation of ZAP-70." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6765-74, 1997