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ZAP70 antibody (zeta-Chain (TCR) Associated Protein Kinase 70kDa) (pTyr493)

Details for Product anti-ZAP70 Antibody No. ABIN362165, Supplier: Log in to see
Antigen
  • 70kDa
  • AI327364
  • MGC80670
  • MGC88962
  • mrtle
  • mur
  • SRK
  • Srk
  • STCD
  • STD
  • TZK
  • ZAP-70
  • zap70
  • ZAP70
Alternatives
anti-Human ZAP70 antibody for Western Blotting
Epitope
pTyr493
50
47
46
25
22
21
21
20
15
15
15
14
12
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
457
171
119
4
4
3
2
2
2
Host
Rabbit
296
141
15
8
3
Clonality
Polyclonal
Conjugate
This ZAP70 antibody is un-conjugated
29
21
16
14
10
7
5
5
4
4
4
4
4
4
4
1
Application
Immunohistochemistry (IHC)
279
162
151
82
62
44
39
38
10
7
5
4
4
3
2
Supplier
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Immunogen Peptide sequence around phosphorylation site of pTyr493 (S-Y-Y (p) -T-A) derived from Human Zap-70. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of Zap-70 only when phosphorylated at tyrosine 493.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Zap-70 (ZAP70 Antibody Abstract)
Background Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.
Molecular Weight 70 kDa
UniProt P43403
Research Area Signaling, Tags/Labels, Immunology, Cancer
Pathways TCR Signaling
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Salomon, Ficarro, Brill, Brinker, Phung, Ericson, Sauer, Brock, Horn, Schultz, Peters: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003

Kong, Dalton, Bubeck Wardenburg, Straus, Kurosaki, Chan: "Distinct tyrosine phosphorylation sites in ZAP-70 mediate activation and negative regulation of antigen receptor function." in: Molecular and cellular biology, Vol. 16, Issue 9, pp. 5026-35, 1996

Chan, Dalton, Johnson, Kong, Wang, Thoma, Kurosaki: "Activation of ZAP-70 kinase activity by phosphorylation of tyrosine 493 is required for lymphocyte antigen receptor function." in: The EMBO journal, Vol. 14, Issue 11, pp. 2499-508, 1995