eIF4EBP1 antibody (pThr45)

Catalog No. ABIN362265

Product Details anti-eIF4EBP1 Antibody

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Binding Specificity: pThr45

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eIF4EBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: The antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr45 (S-T-T (p) -P-G) derived from Human 4E-BP1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-eIF4EBP1 antibody (ABIN362265)

Cao, Gong, Guo, Mietzsch, Kuang, Kwartler, Safi, Estrera, Gambello, Milewicz: "Thoracic aortic disease in tuberous sclerosis complex: molecular pathogenesis and potential therapies in Tsc2+/- mice." in: Human molecular genetics, Vol. 19, Issue 10, pp. 1908-20, (2010) (PubMed).

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: 4E-BP1 (EIF4EBP1 Products)

Synonyms: 4E-BP1 antibody, 4EBP1 antibody, BP-1 antibody, PHAS-I antibody, 4e-bp1 antibody, AA959816 antibody, 153432_at antibody, 43-BP antibody, 4E-BP antibody, 4EBP antibody, 4e-bp antibody, 4eBP antibody, 4ebp antibody, BcDNA.HL08053 antibody, BcDNA:HL08053 antibody, CG8846 antibody, CT8705 antibody, Dm 4E-BP antibody, Dmel\\CG8846 antibody, Phas1 antibody, Thor/EIF4EBP antibody, anon-WO0172774.158 antibody, d4E-BP antibody, d4E-BP1 antibody, d4EBP antibody, d4e-bp antibody, d4eBP antibody, dthor antibody, eIF4E-BP antibody, l(2)06270 antibody, l(2)k07736 antibody, l(2)k13517 antibody, pp20 antibody, thor antibody, EIF4EBP1 antibody, fc04h09 antibody, wu:fc04h09 antibody, zgc:64137 antibody, eukaryotic translation initiation factor 4E binding protein 1 antibody, CG8846 gene product from transcript CG8846-RA antibody, EIF4EBP1 antibody, Eif4ebp1 antibody, Thor antibody, eif4ebp1 antibody

Background: 4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. Published Papers Jiumei Cao

Molecular Weight: 18 kDa

NCBI Accession: NP_004086

UniProt: Q13541

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling