Eukaryotic Translation Initiation Factor 2 alpha Kinase 2 (EIF2AK2) (pThr446) antibody

Antigen: Eukaryotic Translation Initiation Factor 2 alpha Kinase 2 (EIF2AK2)

Synonyms: PKR, PRKR, EIF2AK1, MGC126524

Epitope: pThr446

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Catalog no.: ABIN362357

Additional Information

Alternative name: PKR (Phospho-Thr446)

Swiss-Prot: P19525

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from humanPKR around the phosphorylation site of threonine 446 (K-R-T P -R-S).

Isotype: IgG  (Matching secondary antibodies)

Specificity: PKR (Phospho-Thr446) (Human) antibody detects endogenous levels of PKR only whenphosphorylated at threonine 446.

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200

Concentration: 50µg/50µl

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage: Store at -20°C. Avoid repeat freeze-thaw cycles.

Restrictions: For Research Use only