SHP1 antibody (pTyr536)

Catalog No. ABIN362407

This Rabbit Polyclonal antibody specifically detects SHP1 in IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SHP1 antibody (pTyr536) (ABIN362407)

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHP1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SHP1 Antibody

Binding Specificity: pTyr536

Specificity: The antibody detects endogenous level of SHP-1 only when phosphorylated at tyrosine 536.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr536 (S-E-Y (p) -G-N) derived from Human SHP-1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for SHP1

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Alternative Name: SHP-1

Background: Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.

Molecular Weight: 68 kDa

NCBI Accession: NP_002822

UniProt: P29350

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling