Cyclin E1 antibody (pThr395)
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- Target See all Cyclin E1 (CCNE1) Antibodies
- Cyclin E1 (CCNE1)
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Binding Specificity
- pThr395
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Cyclin E1 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC)
- Specificity
- The antibody detects endogenous level of Cyclin E1 only when phosphorylated at threonine 395.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- Peptide sequence around phosphorylation site of pThr395 (L-L-T (p) -P-P) derived from Human Cyclin E1 Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- Isotype
- IgG
- Top Product
- Discover our top product CCNE1 Primary Antibody
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- Application Notes
- Western blotting: 1:1000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- Target
- Cyclin E1 (CCNE1)
- Alternative Name
- Cyclin E1 (CCNE1 Products)
- Synonyms
- CCNE1 antibody, ccne antibody, cyclinE antibody, CCNE antibody, AW538188 antibody, CycE1 antibody, CYCLE antibody, Ccne antibody, cyce2 antibody, cycE3 antibody, cyce antibody, fa18e07 antibody, wu:fa18e07 antibody, cyclin E1 antibody, cyclin E1 L homeolog antibody, cyclin E1 S homeolog antibody, CCNE1 antibody, ccne1 antibody, Ccne1 antibody, ccne1.L antibody, ccne1.S antibody
- Background
- The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.
- Molecular Weight
- 48 kDa
- NCBI Accession
- NP_001229
- UniProt
- P24864
- Pathways
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
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