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GSK3 alpha antibody (Glycogen Synthase Kinase 3 alpha) (AA 19-23)

Details for Product anti-GSK3a Antibody No. ABIN362496, Supplier: Login to see
Antigen
  • GSK3A
  • CG5182
  • Dmel\\CG5182
  • GSK3a
  • 2700086H06Rik
  • xgsk3
  • GSK-3[a]
  • GSK-3[b]
  • GSK3
  • gsk3a
Alternatives
anti-Human GSK3 alpha antibody for Western Blotting
Epitope
AA 19-23
67
46
26
13
10
9
8
6
6
5
5
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
265
120
92
6
3
2
2
2
1
1
Host
Rabbit
215
79
4
Clonality
Polyclonal
Conjugate
This GSK3 alpha antibody is un-conjugated
20
11
10
6
6
6
4
3
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
239
119
118
38
37
31
15
12
6
3
2
1
Supplier
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Immunogen Peptide sequence around AA 19-23 (T-S-S-F-A) derived from Human GSK3a. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total GSK3a protein. and does not detect GSK-3b protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Alternative Name GSK3alpha (GSK3a Antibody Abstract)
Background Implicated in the hormonal control of several regulatory proteins including glycogen synthase,
Molecular Weight 51 kDa
NCBI Accession NP_063937
UniProt P49840
Pathways PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Welsh, Wilson, Proud: "GSK3: a SHAGGY frog story." in: Trends in cell biology, Vol. 6, Issue 7, pp. 274-9, 2004 (PubMed).

Koivisto, Alavian, Hakkinen et al.: "Glycogen synthase kinase-3 regulates formation of long lamellipodia in human keratinocytes." in: Journal of cell science, Vol. 116, Issue Pt 18, pp. 3749-60, 2003 (PubMed).

Srivastava, Pandey: "Potential mechanism(s) involved in the regulation of glycogen synthesis by insulin." in: Molecular and cellular biochemistry, Vol. 182, Issue 1-2, pp. 135-41, 1999 (PubMed).

Cross, Alessi, Cohen et al.: "Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B." in: Nature, Vol. 378, Issue 6559, pp. 785-9, 1996 (PubMed).