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MAP2K6 antibody (Mitogen-Activated Protein Kinase Kinase 6) (AA 205-209)

Details for Product anti-MAP2K6 Antibody No. ABIN362761, Supplier: Log in to see
Antigen
  • ANQ1
  • ARABIDOPSIS NQK1
  • ARABIDOPSIS THALIANA MAP KINASE KINASE 6
  • ATMKK6
  • map2k3
  • MAP kinase kinase 6
  • MAPKK6
  • MEK6
  • MIK19.2
  • MIK19_2
  • MKK3
  • Mkk6
  • MKK6
  • PRKMK6
  • Prkmk6
  • SAPKK-3
  • SAPKK3
  • zMKK3
Alternatives
anti-Human MAP2K6 antibody for Western Blotting
Epitope
AA 205-209
50
34
15
9
6
5
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
175
103
81
3
2
2
Host
Rabbit
163
33
2
Clonality
Polyclonal
Conjugate
This MAP2K6 antibody is un-conjugated
5
5
5
3
3
3
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
160
73
71
43
27
23
18
13
8
3
2
1
Supplier
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Immunogen Peptide sequence around AA 205-209 (V-D-S-V-A) derived from Human MKK6. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total MKK6 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Alternative Name MKK6 (MAP2K6 Antibody Abstract)
Background MEK6 is a member of MAPKK protein kinase family. By using degenerate oligonucleotide primers from the conserved kinase domains of MKK3 and MKK4 two human cDNAs and 1 murine cDNA encoding closely related proteins of the MKK family were cloned. The two human clones appear to be different isoforms of the same gene generated by differential splicing: the shorter clone was designated MKK6, encodes a 278-amino acid protein, while the longer clone, designated MKK6b, encodes a 334-amino acid protein. MKK6 is about 80% identical to MKK3 and 40% identical to MKK4. 1.7-kb human MKK6 transcript is highly expressed in skeletal muscle, while an MKK6b-specific probe detected mRNA bands of 1.8, 2.4, and 4.5 kb that are enriched in heart, skeletal muscle, pancreas and liver. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase . MEK6 phosphorylates and activates p38 in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAPK mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
Molecular Weight 41 kDa
NCBI Accession NP_002749
UniProt P52564
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Wang, Chen, Liao, Deng, Zhou, Huang, Sun: "Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3389-403, 2002 (PubMed).

Raingeaud, Whitmarsh, Barrett, Dérijard, Davis: "MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway." in: Molecular and cellular biology, Vol. 16, Issue 3, pp. 1247-55, 1996 (PubMed).