The antibody detects endogenous level of total Paxillin protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
Peptide sequence around AA 29-33 (T-P-Y-S-Y) derived from Human Paxillin. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions