CTLA4
Reactivity: Human, Mouse, Rat
FACS, WB, ELISA, IHC (p), IF (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow Cytometry. Immunoprecipitation. In vitro and in vivo blocking of CTLA-4 action by soluble antibody. Promotion of in vitro CTLA-4 signalling by cross-linked antibody. See Ref.1 for more details. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
PBS containing no preservatives.
Preservative
Without preservative
Storage
-20 °C
Storage Comment
Store the antibody undiluted (in aliquous) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
CTLA4
(Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
CD152, also known as CTLA-4, is homologous to the costimulatory molecule CD28. (Ref.1,2) Like CD28, the CD152 Molecule binds the family of B7 counter-receptors (CD80/B7-1 and CD86/B7-2) on antigen-presenting cells. (Ref.3) CTLA-4 is not expressed on resting T lymphocytes but is induced 2-3 days following T-cell stimulation via the T cell receptor (TCR) complex. (Ref.1,3,4) Whereas CD28 provides a positive costimulatory signal, CD152/CTLA-4 is a negative regulator of T-cell activation, possibly by inhibiting tyrosine kinase signalling through the TCR through its association with tyrosine phosphatases such as SHP-2. (Ref.1,5,6)Synonyms: CTLA-4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4