TLR4 antibody (Toll-Like Receptor 4)

Details for Product anti-TLR4 Antibody No. ABIN371647, Supplier: Log in to see
Antigen
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll-like receptor 4
  • TLR4
  • Tlr4
Alternatives
anti-Human TLR4 antibody for Antagonist
Reactivity
Human
500
258
142
75
51
51
43
26
12
10
4
2
2
1
1
Host
Mouse
292
251
60
9
Clonality (Clone)
Monoclonal ()
Conjugate
This TLR4 antibody is un-conjugated
47
42
40
19
15
13
12
11
10
8
8
8
8
7
7
6
6
6
6
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS)
365
293
136
134
123
107
98
87
34
27
26
24
21
16
12
8
7
4
4
3
3
2
2
1
1
1
Options
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Clone 1-00E-011
Isotype IgG2a
Characteristics Synonyms: Toll-like receptor 4
Purification Purified
Alternative Name CD284 / TLR4 (TLR4 Antibody Abstract)
Background The Toll-like receptor (TLR) family is comprised of Type I transmembrane proteins characterized by an extracellular leucine-rich domain and a cytoplasmic tail that contains a conserved region call the Toll/IL-1 (TIR) domain. First discovered in Drosophila, TLRs recognize specific molecular patterns that are present in microbial components and respond to these components in order eliminate or limit invading microbes. To date, ten mammalian homologs of TLRs have been described. TLR4 is involved in the recognition of lipopolysaccharide (LPS), an abundant glycolipid of Gram-negative bacteria. Through its association with MD-2 and CD14, TLR4 recognizes and initiates multiple intracellular signaling events such as the activation of NF-kB leading to the ultimate synthesis and release of a number of proinflammatory mediators including interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-alpha). TLR4 has also been associated in the recognition of and lipocheic acid (LTA), Hsp60 and Hsp70 (Ref.1-10)Synonyms: Toll-like receptor 4
UniProt O00206
Research Area Signaling, Immunology, Inflammation
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades
Application Notes ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer 100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Habich, Baumgart, Kolb, Burkart: "The receptor for heat shock protein 60 on macrophages is saturable, specific, and distinct from receptors for other heat shock proteins." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 2, pp. 569-76, 2002 (PubMed).

Asea, Rehli, Kabingu, Boch, Bare, Auron, Stevenson, Calderwood: "Novel signal transduction pathway utilized by extracellular HSP70: role of toll-like receptor (TLR) 2 and TLR4." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15028-34, 2002 (PubMed).

Dziarski, Gupta: "Role of MD-2 in TLR2- and TLR4-mediated recognition of Gram-negative and Gram-positive bacteria and activation of chemokine genes." in: Journal of endotoxin research, Vol. 6, Issue 5, pp. 401-5, 2001 (PubMed).

Lien, Means, Heine, Yoshimura, Kusumoto, Fukase, Fenton, Oikawa, Qureshi, Monks, Finberg, Ingalls, Golenbock: "Toll-like receptor 4 imparts ligand-specific recognition of bacterial lipopolysaccharide." in: The Journal of clinical investigation, Vol. 105, Issue 4, pp. 497-504, 2000 (PubMed).

Jiang, Akashi, Miyake, Petty: "Lipopolysaccharide induces physical proximity between CD14 and toll-like receptor 4 (TLR4) prior to nuclear translocation of NF-kappa B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3541-4, 2000 (PubMed).

Ohashi, Burkart, Flohé, Kolb: "Cutting edge: heat shock protein 60 is a putative endogenous ligand of the toll-like receptor-4 complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 2, pp. 558-61, 2000 (PubMed).

Chow, Young, Golenbock, Christ, Gusovsky: "Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction." in: The Journal of biological chemistry, Vol. 274, Issue 16, pp. 10689-92, 1999 (PubMed).

Shimazu, Akashi, Ogata, Nagai, Fukudome, Miyake, Kimoto: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999 (PubMed).

Zhang, Kirschning, Mancinelli, Xu, Jin, Faure, Mantovani, Rothe, Muzio, Arditi: "Bacterial lipopolysaccharide activates nuclear factor-kappaB through interleukin-1 signaling mediators in cultured human dermal endothelial cells and mononuclear phagocytes." in: The Journal of biological chemistry, Vol. 274, Issue 12, pp. 7611-4, 1999 (PubMed).

Takeuchi, Hoshino, Kawai, Sanjo, Takada, Ogawa, Takeda, Akira: "Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components." in: Immunity, Vol. 11, Issue 4, pp. 443-51, 1999 (PubMed).